Journal: Frontiers in Medicine

Article Title: Deciphering CHFR Role in Pancreatic Ductal Adenocarcinoma

doi: 10.3389/fmed.2021.720128

Figure Lengend Snippet: CHFR methylation analysis of human samples, borderline (A–F) and resectable cohort (G–L) . Methylation-Specific PCR (left) and Pyrosequencing Assay (right). T (tumoral tissue) and A (Adjacent tissue). *Statistically significant differences ( p < 0.05). Columns represents mean and error bars correspond to standard deviations.

Article Snippet: It was in the latter cohort where we were able to establish the utility of this methylation pattern as an independent prognostic for OS, similar to that which was previously described by the Human Protein Atlas (HPA) in kidney carcinoma (available at http://www.proteinatlas.org ).

Techniques: Methylation, Pyrosequencing Assay

Journal: Frontiers in Medicine

Article Title: Deciphering CHFR Role in Pancreatic Ductal Adenocarcinoma

doi: 10.3389/fmed.2021.720128

Figure Lengend Snippet: Correlation of CHFR methylation with clinical outcomes in resectable patients. Kaplan-meier curve representing OS for resectable cohort and dichotomized by CpG-2 median methylation percentage.

Article Snippet: It was in the latter cohort where we were able to establish the utility of this methylation pattern as an independent prognostic for OS, similar to that which was previously described by the Human Protein Atlas (HPA) in kidney carcinoma (available at http://www.proteinatlas.org ).

Techniques: Methylation

Journal: Nature Communications

Article Title: Alveolar epithelial cells mitigate neutrophilic inflammation in lung injury through regulating mitochondrial fatty acid oxidation

doi: 10.1038/s41467-024-51683-1

Figure Lengend Snippet: a Representative results of immunohistochemistry staining for CPT1a, long-chain acyl-CoA dehydrogenase (LCAD), and cytokeratin (CK) using surgical sections from control and diffuse alveolar damage (DAD) donors (scale bar = 50 μm). b , c The alveolar epithelial levels of CPT1a ( b ) and LCAD ( c ) are semi-quantified using the H scores (control n = 18, DAD n = 27; the line indicates median; two-sided p values are calculated by Mann–Whitney U tests). d Expression of fatty acid β-oxidation pathway (GO: 0006635) in AT2 cells in ARDS is analyzed using human lung single-cell RNA sequencing datasets from people with fatal COVID-19 and controls (GSE171524, GSE171668). Expression score for each cell is calculated by averaging the expression of the gene involved in the fatty acid β-oxidation pathway, and the comparison between groups is exhibited using violin plots. Two-sided p values are calculated using Mann–Whitney U tests. e AT2 cells from Sftpc CreERt2+/ − mice instilled with LPS or ddH 2 O are isolated for extracellular flux analyses using palmitate alone or after etomoxir pretreatment (Oligo oligomycin, Rot rotenone, AA antimycin A). f Oxygen consumption rates (OCRs) for ATP production are then calculated (palmitate alone, n = 8 replicates per group; palmitate with etomoxir pretreatment, n = 10 replicates per group; both are representative data from two independent experiments). e , f Data are represented as mean ± SEM, and f two-sided p values are calculated by unpaired Student’s t-tests.

Article Snippet: According to The Human Protein Atlas , CPT1a is the main CPT1 isoform expressed in human lungs, while CPT1b and CPT1c are not expressed.

Techniques: Immunohistochemistry, Staining, Control, MANN-WHITNEY, Expressing, RNA Sequencing, Comparison, Isolation

Journal: Nature Communications

Article Title: Alveolar epithelial cells mitigate neutrophilic inflammation in lung injury through regulating mitochondrial fatty acid oxidation

doi: 10.1038/s41467-024-51683-1

Figure Lengend Snippet: a The immunoblot shows CPT1a and LCAD expression in different cellular populations selected from single-cell suspensions using anti-CD45 and anti-EpCAM (representative data of two independent experiments). b A scheme showing the generation of transgenic mice with tamoxifen-inducible AT2 cell-specific Cpt1a deletion. c Immunoblots of control and Cpt1a − / − AT2 cells isolated 6 weeks after tamoxifen injection (n = 3 mice per group; representative data of two independent experiments). d Intracellular levels of carnitine (C0), palmitoylcarnitine (C16), and stearoylcarnitine (C18) are measured for calculating C0/(C16 + C18) ratio in AT2 cells (n = 5 mice per group; representative data of two independent experiments). e – h Extracellular flux analyses ( e ) with palmitate alone (Oligo oligomycin, Rot rotenone, AA antimycin A) or g after etomoxir pretreatment in AT2 cells, and oxygen consumption rates (OCRs) f , h are calculated (palmitate, n = 9 replicates per group; palmitate/etomoxir, control n = 7 replicates, Cpt1a − / − n = 8 replicates; representative data of two independent experiments). d – h Data are represented as mean ± SEM, and d , f , h two-sided p values are calculated by unpaired Student’s t-tests.

Article Snippet: According to The Human Protein Atlas , CPT1a is the main CPT1 isoform expressed in human lungs, while CPT1b and CPT1c are not expressed.

Techniques: Western Blot, Expressing, Transgenic Assay, Control, Isolation, Injection

Journal: Nature Communications

Article Title: Alveolar epithelial cells mitigate neutrophilic inflammation in lung injury through regulating mitochondrial fatty acid oxidation

doi: 10.1038/s41467-024-51683-1

Figure Lengend Snippet: a Intracellular ATP levels in the control and Cpt1a − / − AT2 cells (n = 5 mice per group). b , c Extracellular flux analyses ( b ) with glucose (Glu), pyruvate (Pyr), and glutamine (Gln) in AT2 cells, and OCRs ( c ) are calculated (n = 9 per group; representative data of two independent experiments). d , e Glycolysis stress tests ( d ) in AT2 cells (2DG, 2-deoxyglucose), and e extracellular acidification rates (ECARs) are calculated (n = 10 replicates per group; representative data of two independent experiments). f – k Extracellular flux analyses with ( f ) glutamine alone, h pyruvate alone, or j both pyruvate and glutamine in AT2 cells, and OCRs for ATP production using ( g ) glutamine alone (control n = 10 replicates, Cpt1a − / − n = 9 replicates), i pyruvate alone (n = 10 replicates per group), or k both pyruvate and glutamine (control, n = 9 replicates, Cpt1a − / − , n = 7 replicates) are calculated ( f – k ), all are representative data of two independent experiments. l A cartoon illustrating the metabolic adaptations in Cpt1a − / − AT2 cells (TCA tricarboxylic acid, OXPHOS oxidative phosphorylation). a – k Data are represented as mean ± SEM, and a , c , e , g , I , k two-sided p values are calculated by unpaired Student’s t-tests.

Article Snippet: According to The Human Protein Atlas , CPT1a is the main CPT1 isoform expressed in human lungs, while CPT1b and CPT1c are not expressed.

Techniques: Control, Phospho-proteomics

Journal: Nature Communications

Article Title: Alveolar epithelial cells mitigate neutrophilic inflammation in lung injury through regulating mitochondrial fatty acid oxidation

doi: 10.1038/s41467-024-51683-1

Figure Lengend Snippet: a , b Flow cytometric analyses ( a ) evaluating phospholipid synthesis in Cpt1a − / − and control MLE12 cells. The geometric mean fluorescent intensity (MFI) of phospholipid labeling ( b ) represents phospholipid synthesis activity (n = 3 replicates per group; representative data of two independent experiments). c – e Immunoblots ( c ) confirming CPT1a overexpression in Cpt1a OE MLE12 cells, generated through lentiviral transduction (control MLE12 cells generated through lentiviral transduction of empty construct; representative data of two independent experiments). d Flow cytometric analyses evaluating phospholipid synthesis assay of Cpt1a OE and control MLE12 cells, and I phospholipid synthesis activity is compared (n = 3 replicates per group; representative data of two independent experiments). f , g Flow cytometric analyses ( f ) evaluating phospholipid synthesis in AT2 cells, and the phospholipid synthesis activity ( g ) is compared (n = 3 replicates per group; representative data of two independent experiments). h , i Intracellular contents of PC and phosphatidylglycerol (PG) species in AT2 cells, and data of the top 5 abundant h PC and i PG species are shown (n = 12 mice per group; data represent results of two independent experiments). j , k The contents of PC and PG species in bronchoalveolar lavage fluid (BALF) samples, and data of the top five abundant j PC and k PG species are demonstrated (control mice n = 10, Cpt1a iΔAT2 mice n = 9; data represent results of two independent experiments). b , e , g , and h – k Data are represented as mean ± SEM, and b , e , g – k two-sided p values are calculated by unpaired Student’s t-tests ( h – k , only p values less than 0.05 are shown).

Article Snippet: According to The Human Protein Atlas , CPT1a is the main CPT1 isoform expressed in human lungs, while CPT1b and CPT1c are not expressed.

Techniques: Control, Labeling, Activity Assay, Western Blot, Over Expression, Generated, Transduction, Construct

Journal: Nature Communications

Article Title: Alveolar epithelial cells mitigate neutrophilic inflammation in lung injury through regulating mitochondrial fatty acid oxidation

doi: 10.1038/s41467-024-51683-1

Figure Lengend Snippet: a , b At 24 h after intratracheal LPS instillation, flow cytometric analyses ( a ) are performed to evaluate ( b ) the percentages of various immune cells, including alveolar macrophages (CD11c + SiglecF + ), neutrophils (CD11c − Ly6G + ), eosinophils (CD11c − Ly6G − CD3 − SiglecF + ), and T lymphocytes (CD11c − Ly6G − CD3 + SiglecF − ), in the CD45.2+ cell population in bronchoalveolar lavage fluid (BALF) (control mice n = 20, Cpt1a iΔAT2 mice n = 16; results of two independent experiments). c – h BALF samples are collected at 24 h ( c , d , g ) and 72 h ( e , f , h ). The c , e total cell number and the level of d , f protein and g , h various cytokines/chemokines, including CXCL1, CXCL2, tumor necrosis factor (TNF) α, and interleukin (IL) 6, in BALF are measured (data at 24 h: control mice, c , d n = 8 g n = 4 for ddH 2 O, c , d , g n = 47 for LPS; Cpt1a iΔAT2 mice, c , d n = 7 g n = 3 for ddH 2 O, LPS c , d , g n = 35 for LPS; results of four independent experiments; e , f , h data at 72 h: control mice, n = 4 for ddH 2 O, n = 27 for LPS; Cpt1a iΔAT2 mice, n = 4 for ddH 2 O, n = 33 for LPS; results of two independent experiments). b , c – h Data are represented as mean ± SEM, and two-sided p values are calculated by ( b ) unpaired Student’s t-tests or c – h one-way ANOVA with Bonferroni correction.

Article Snippet: According to The Human Protein Atlas , CPT1a is the main CPT1 isoform expressed in human lungs, while CPT1b and CPT1c are not expressed.

Techniques: Control

Journal: Nature Communications

Article Title: Alveolar epithelial cells mitigate neutrophilic inflammation in lung injury through regulating mitochondrial fatty acid oxidation

doi: 10.1038/s41467-024-51683-1

Figure Lengend Snippet: a A cartoon illustrating the investigation for the link between altered baseline alveolar PG compositions in Cpt1a iΔAT2 mice and the immuno-modulation in ALI. b – f Measuring levels of free fatty acid (FFA), PG, and lysophosphatidylglycerol (LPG) species in bronchoalveolar lavage fluid (BALF) samples. Lipidomic analyses are performed to measure PG and LPG compositions in BALF, and the total measured PG species ( b ), the top 5 abundant PG species ( c ) and the ratios of LPG to PG ( d ) in BALF are compared (control mice, n = 4 for ddH 2 O, n = 24 for LPS; Cpt1a iΔAT2 mice, n = 4 for ddH 2 O, n = 20 for LPS; results of two independent experiments). e Free fatty acid (FFA) levels are measured in BALF samples at 24 h after instillation (control mice, n = 4 for ddH 2 O, n = 23 for LPS; Cpt1a iΔAT2 mice, n = 3 for ddH 2 O, n = 15 for LPS; results of two independent experiments). f The contents of the top 5 abundant LPG species in BALF samples at 24 h after LPS instillation are demonstrated and compared (control mice, n = 24, Cpt1a iΔAT2 mice, n = 20; results of two independent experiments). g An experimental schema showing intratracheal instillation of LPS with solvent, PG(32:0)_16:0 (~1.5 mM), or PG(34:1)_18:1 (~1.5 mM) to induce ALI with obliteration of alveolar phospholipidomic differences of specific PG species at baseline. BALF samples are collected 24 h after the induction of ALI. h – l The BALF ( h ) cell number and levels of i protein, j CXCL1, k CXCL2, and l TNFα are measured and compared (control mice, n = 16 for solvent, n = 10 for PG(32:0)_16:0, and n = 10 for PG(34:1)_18:1; Cpt1a iΔAT2 mice, n = 16 for solvent, n = 10 for PG(32:0)_16:0, and n = 10 for PG(34:1)_18:1; results of three independent experiments). b – f and h – l Data are represented as mean ± SEM, and two-sided p values are calculated by ( e ) one-way ANOVA with Bonferroni correction or b – d , f , h – l unpaired Student’s t-tests ( c and f , only p values less than 0.05 are shown).

Article Snippet: According to The Human Protein Atlas , CPT1a is the main CPT1 isoform expressed in human lungs, while CPT1b and CPT1c are not expressed.

Techniques: Control, Solvent

Journal: Nature Communications

Article Title: Alveolar epithelial cells mitigate neutrophilic inflammation in lung injury through regulating mitochondrial fatty acid oxidation

doi: 10.1038/s41467-024-51683-1

Figure Lengend Snippet: a At 24 h after LPS instillation to control tdTomato − AT2 mice, cryosections of lungs are obtained for staining of CD45, CXCL1, and CXCL2 and confocal microscopy (representative data from n = 7 mice; arrow, chemokine in AT2 cells; arrowhead, chemokine in immune cells; scale bar = 20 μm). b , c RNA sequencing (RNAseq) in control AT2 cells after LPS instillation (24 h). A functional enrichment map ( b ) plotted using genes differently expressed (an adjusted p < 0.01 and a fold change > 2) at baseline and in ALI. c The expression levels of cytokine and chemokine genes included in cytokine production (GO: 0001816) and leukocyte migration (GO: 0050900) annotations are demonstrated in transcript per million (TPM) (n = 4 mice for each group). d Heatmaps comparing the expression of genes involved in cytokine production and leukocyte migration annotations in control and Cpt1a − / − AT2 cells (n = 4 mice for each group). The expression levels of e Cxcl1 and f Cxcl2 in AT2 cells are compared across the groups. g , h Quantitative polymerase chain reactions (qPCRs) measuring g Cxcl1 and h Cxcl2 expression in AT2 cells at 24 h after LPS instillation, using Tbp for normalization (data analyzed by 2 − ΔΔCt method; control n = 10 mice, Cpt1a − / − n = 11 mice; results of four independent experiments). i After 6 h of tumor necrosis factor (TNF) α (2 nM) activation along with CXCL2 or not, Cxcl2 expression in bone marrow derived neutrophils is measured by qPCR (n = 4 replicates per group; representative data of three independent experiments). j , k qPCRs measuring Cxcl1 and Cxcl2 expression in alveolar neutrophils in ALI (control mice, n = 17, Cpt1a iΔAT2 mice, n = 14; results of three independent experiments). c , e – k Data are represented as mean ± SEM, and two-sided p values are calculated by ( e , f ) linear modeling and empirical Bayes moderation, adjusted through the Benjamini–Hochberg procedures, g , h , j , k unpaired Student’s t-tests or i one-way ANOVA with Bonferroni correction. b – f The details of RNAseq analyses are described in “Methods” section.

Article Snippet: According to The Human Protein Atlas , CPT1a is the main CPT1 isoform expressed in human lungs, while CPT1b and CPT1c are not expressed.

Techniques: Control, Staining, Confocal Microscopy, RNA Sequencing, Functional Assay, Expressing, Migration, Activation Assay, Derivative Assay

Journal: PeerJ

Article Title: NCBP2 predicts the prognosis and the immunotherapy response of cancers: a pan-cancer analysis

doi: 10.7717/peerj.19050

Figure Lengend Snippet: (A) Genomic alteration profiles of NCBP1, NCBP2, NCBP3, NCBP2-AS1, NCBP2AS2 and NCBP2L in TCGA pan-cancer cohort. (B–C) Kaplan–Meier survival analysis showed OS (B) and DFS (C) in all pan-cancer patients divided by NCBP2 altered or unaltered groups. (D) The genomic alteration of NCBP2 in TCGA pan-cancer atlas, including mutation, amplification, deep deletion and multiple alterations.

Article Snippet: Dataset. https://doi.org/10.6084/m9.figshare.28399436.v1 IMvigor210: http://research-pub.gene.com GSE91061: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE91061 Human Protein Atlas, NCBP2: https://www.proteinatlas.org/ENSG00000114503-NCBP2/subcellular CCLE database (modified) and GDSC database (modified): li, shichao (2025).

Techniques: Mutagenesis, Amplification

Journal: PeerJ

Article Title: NCBP2 predicts the prognosis and the immunotherapy response of cancers: a pan-cancer analysis

doi: 10.7717/peerj.19050

Figure Lengend Snippet: (A) The relative mRNA expression level of NCBP2 between tumor and normal tissues in pan-cancer from TCGA cohort. (B) The expression of NCBP2 in several cancer cell lines, including U-251, A-431, U2-OS, PC3 and MCF7 were detected by immunofluorescent staining in Human Protein Atlas. (C–F) The protein expression of NCBP2 in LUSC (C), KIRP (D), PAAD (E) and KIRC (F) and paired normal tissues were detected by immunohistochemical stain. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Dataset. https://doi.org/10.6084/m9.figshare.28399436.v1 IMvigor210: http://research-pub.gene.com GSE91061: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE91061 Human Protein Atlas, NCBP2: https://www.proteinatlas.org/ENSG00000114503-NCBP2/subcellular CCLE database (modified) and GDSC database (modified): li, shichao (2025).

Techniques: Expressing, Staining, Immunohistochemical staining

Journal: PeerJ

Article Title: NCBP2 predicts the prognosis and the immunotherapy response of cancers: a pan-cancer analysis

doi: 10.7717/peerj.19050

Figure Lengend Snippet: (A) The correlation between NCBP2 and patients’ OS were shown by forest plot. (B–G) Kaplan–Meier survival analysis for patients with specific cancers (UCEC, PAAD, LIHC, KIRC, KICH and KIRP) base on the expression of NCBP2. (H–M) Multivariate cox regression analyses evaluated the prognostic independence of NCBP2 and clinicopathological features regarding OS of UCEC, PAAD, LIHC, KIRC, KICH and KIRP in TCGA datasets.

Article Snippet: Dataset. https://doi.org/10.6084/m9.figshare.28399436.v1 IMvigor210: http://research-pub.gene.com GSE91061: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE91061 Human Protein Atlas, NCBP2: https://www.proteinatlas.org/ENSG00000114503-NCBP2/subcellular CCLE database (modified) and GDSC database (modified): li, shichao (2025).

Techniques: Expressing

Journal: PeerJ

Article Title: NCBP2 predicts the prognosis and the immunotherapy response of cancers: a pan-cancer analysis

doi: 10.7717/peerj.19050

Figure Lengend Snippet: Differential expressed genes (DEGs) were analyzed between the top and bottom 30% NCBP2 expression subgroup for each cancer in TCGA, following hallmarks gene set enrichment analysis (GSEA) of those DEGs to investigate the NCBP2-associated cancer processes. The size of the circle represents the false discovery rate (FDR) adjusted P value of each cancer enrichment item, and the color represents the normalized enrichment score (NES) of each enrichment item.

Article Snippet: Dataset. https://doi.org/10.6084/m9.figshare.28399436.v1 IMvigor210: http://research-pub.gene.com GSE91061: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE91061 Human Protein Atlas, NCBP2: https://www.proteinatlas.org/ENSG00000114503-NCBP2/subcellular CCLE database (modified) and GDSC database (modified): li, shichao (2025).

Techniques: Expressing

Journal: PeerJ

Article Title: NCBP2 predicts the prognosis and the immunotherapy response of cancers: a pan-cancer analysis

doi: 10.7717/peerj.19050

Figure Lengend Snippet: (A) Heatmap showing the correlation of NCBP2 expression and stromal score, immune score, and ESTIMATE score in pan-cancer. Positive correlation in red and negative correlation in blue. (B) Heatmap showing the correlation of NCBP2 and infiltration levels of immune cells including CD4+ T cells, CAF, progenitor, Endo, Eos, HSC, Tfh, gdT, NKT, regulatory T cells (Tregs), B cells, neutrophils, monocytes, macrophages, dendritic cells, NK cells, Mast cells and CD8+ T cells in cancers. Positive correlation in red and negative correlation in blue. * p < 1.05; ** p < 0.01.

Article Snippet: Dataset. https://doi.org/10.6084/m9.figshare.28399436.v1 IMvigor210: http://research-pub.gene.com GSE91061: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE91061 Human Protein Atlas, NCBP2: https://www.proteinatlas.org/ENSG00000114503-NCBP2/subcellular CCLE database (modified) and GDSC database (modified): li, shichao (2025).

Techniques: Expressing

Journal: PeerJ

Article Title: NCBP2 predicts the prognosis and the immunotherapy response of cancers: a pan-cancer analysis

doi: 10.7717/peerj.19050

Figure Lengend Snippet: (A) The Spearman correlation heatmap shows the correlation between the expression of NCBP2 and twenty-six immunomodulatory genes in pan-cancer. Red represents positive correlation and blue represents negative correlation. (B–C) Correlations between NCBP2 and tumor mutation burden (TMB) (B) and microsatellite instability (MSI) (C) in pan-cancer. (D) The proportion of patients who receive immunotherapy in the low- and high- NCBP2 subgroup in the IMvigor210 cohort (bladder cancer). (E) Kaplan–Meier curve of low- and high- NCBP2 subgroup in the IMvigor210 cohort. (F) The proportion of patients who receive immunotherapy in the low- and high- NCBP2 subgroup in the GSE91061 cohort (melanoma). (G) Kaplan–Meier curve of low- and high- NCBP2 subgroup in the GSE91061 cohort. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Dataset. https://doi.org/10.6084/m9.figshare.28399436.v1 IMvigor210: http://research-pub.gene.com GSE91061: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE91061 Human Protein Atlas, NCBP2: https://www.proteinatlas.org/ENSG00000114503-NCBP2/subcellular CCLE database (modified) and GDSC database (modified): li, shichao (2025).

Techniques: Expressing, Mutagenesis

Journal: PeerJ

Article Title: NCBP2 predicts the prognosis and the immunotherapy response of cancers: a pan-cancer analysis

doi: 10.7717/peerj.19050

Figure Lengend Snippet: (A) Heatmap exhibited the relationship between NCBP2 and stemness indices across cancers. Positive correlation in red and negative correlation in blue. (B) (upper) The relationship between NCBP2 and drug sensitivity calculated by Spearman algorithm. The color of each column represents the p value, whereas the height of each column represents the correlation coefficient. Rs represents the drug sensitivity correlated with the NCBP2 expression. (lower) Dot plot visualized the signal pathways targeted by drugs which were sensitivity (blue) or resistant (red) to the NCBP2. The signal pathways were ranked by the frequency of being targeted. * p < 1.05; ** p < 0.01.

Article Snippet: Dataset. https://doi.org/10.6084/m9.figshare.28399436.v1 IMvigor210: http://research-pub.gene.com GSE91061: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE91061 Human Protein Atlas, NCBP2: https://www.proteinatlas.org/ENSG00000114503-NCBP2/subcellular CCLE database (modified) and GDSC database (modified): li, shichao (2025).

Techniques: Expressing

Journal: PeerJ

Article Title: NCBP2 predicts the prognosis and the immunotherapy response of cancers: a pan-cancer analysis

doi: 10.7717/peerj.19050

Figure Lengend Snippet: (A) Correlation analysis between NCBP2 and cancer-related functional states at single cell level according to CancerSEA dataset. (B–D) The association between NCBP2 expression and several biological process in AML (B), UM (C), RB (D). (E–G) t-SNE diagrams were used to visualize NCBP2 expression profiles in AML (E), UM (F), RB (G).

Article Snippet: Dataset. https://doi.org/10.6084/m9.figshare.28399436.v1 IMvigor210: http://research-pub.gene.com GSE91061: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE91061 Human Protein Atlas, NCBP2: https://www.proteinatlas.org/ENSG00000114503-NCBP2/subcellular CCLE database (modified) and GDSC database (modified): li, shichao (2025).

Techniques: Functional Assay, Expressing

Journal: PLoS ONE

Article Title: Activated Leukocyte Cell Adhesion Molecule Expression and Shedding in Thyroid Tumors

doi: 10.1371/journal.pone.0017141

Figure Lengend Snippet: Immunohistochemical reactivity of thyroid tumors.

Article Snippet: These results are in agreement with the annotated immunohistochemical patterns in Human Protein Atlas database ( www.proteinatlas.org ).

Techniques: Immunohistochemical staining